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array ifc  (fluidigm)


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    Structured Review

    fluidigm array ifc
    Array Ifc, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dynamic+array+ifc/96%2E96+Dynamic+Array+IFC+for+Gene+Expression/pm41915123-70-9-12
    Average 95 stars, based on 716 article reviews
    array ifc - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Gene Expression:

    Article Title: A mix of functional amino acids and grape polyphenols promotes the growth of piglets, modulates the gut microbiota in vivo and regulates epithelial homeostasis in intestinal organoids.
    Article Snippet: Weaning is a challenging period for gut health in piglets.. Previous studies showed that dietary supplementations with either amino acids or polyphenols promote piglet growth and intestinal functions, when administered separately.. Thus, we hypothesized that a combination of amino acids and polyphenols could facilitate the weaning transition.

    Article Title: Protective interplay: Mycobacterium tuberculosis diminishes SARS-CoV-2 severity through innate immune priming.
    Article Snippet: The obtained RNA was then utilized in the HighCapacity RNA-to-cDNA kit for cDNA synthesis using SuperScriptTM IV Reverse Transcriptase (Thermo Fisher Scientific), containing a reverse transcriptase with a high-fidelity enzyme following manufacture protocol. .. For Fluidigm Real-Time PCR and Dynamic Array IFC (Integrated Fluidic Circuit) Setup, specific target amplification (STA) was done as per the manufacturer’s recommendations as the initial step (pre-amplification of cDNA) for the Biomark HD system (Standard BioTools, formerly Fluidigm) carried out on the Standard BioTools 48.48 Gene Expression (GE) Dynamic Array integrated fluidic circuit (IFC) (49). ..

    Article Title: Divergent Photoperiodic Responses in Hypothalamic Dio3 Expression and Gonadal Activity Between Offspring and Paternal Brandt's Voles.
    Article Snippet: .. Samples and assays were then loaded into the Dynamic Array IFC (Fluidigm Sciences Inc., Sunnyvale, CA, USA), and gene expression was quantified using SsoFast EvaGreen Supermix (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with the Fluidigm Biomark HD system. .. For Dio3, which was undetectable in the BioMark HD System, relative expression was determined using SYBR Green PCR mix (Applied Biosystems, Foster City, CA, USA) on an Applied Biosystems 7500 (Applied Biosystems, Foster City, CA, USA).

    Article Title: The Early Life Microbiota Is Not a Major Factor Underlying the Susceptibility to Postweaning Diarrhea in Piglets.
    Article Snippet: A total of 500 ng RNA purified with Direct-zol RNA MiniPrep Plus kit (Zymo Research) were retrotranscribed into cDNA with the kit GoScript Reverse Transcription Mix, Random primer (Promega) as described previously (37). .. Gene expression was analyzed by real-time qPCR using Biomark microfluidic system using a Dynamic Array IFC for gene expression (Fluidigm) according to the manufacturer recommendations July/August 2023 Volume 11 Issue 4 10.1128/spectrum.00694-23 10 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 24 J an ua ry 2 02 4 by 1 03 .1 05 .3 4. ..

    Article Title: Divergent Photoperiodic Responses in Hypothalamic Dio3 Expression and Gonadal Activity Between Offspring and Paternal Brandt’s Voles
    Article Snippet: .. Samples and assays were then loaded into the Dynamic Array IFC (Fluidigm Sciences Inc., Sunnyvale, CA, USA), and gene expression was quantified using SsoFast EvaGreen Supermix (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with the Fluidigm Biomark HD system. .. For Dio3 , which was undetectable in the BioMark HD System, relative expression was determined using SYBR Green PCR mix (Applied Biosystems, Foster City, CA, USA) on an Applied Biosystems 7500 (Applied Biosystems, Foster City, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: A mix of functional amino acids and grape polyphenols promotes the growth of piglets, modulates the gut microbiota in vivo and regulates epithelial homeostasis in intestinal organoids.
    Article Snippet: Weaning is a challenging period for gut health in piglets.. Previous studies showed that dietary supplementations with either amino acids or polyphenols promote piglet growth and intestinal functions, when administered separately.. Thus, we hypothesized that a combination of amino acids and polyphenols could facilitate the weaning transition.

    Article Title: Protective interplay: Mycobacterium tuberculosis diminishes SARS-CoV-2 severity through innate immune priming.
    Article Snippet: The obtained RNA was then utilized in the HighCapacity RNA-to-cDNA kit for cDNA synthesis using SuperScriptTM IV Reverse Transcriptase (Thermo Fisher Scientific), containing a reverse transcriptase with a high-fidelity enzyme following manufacture protocol. .. For Fluidigm Real-Time PCR and Dynamic Array IFC (Integrated Fluidic Circuit) Setup, specific target amplification (STA) was done as per the manufacturer’s recommendations as the initial step (pre-amplification of cDNA) for the Biomark HD system (Standard BioTools, formerly Fluidigm) carried out on the Standard BioTools 48.48 Gene Expression (GE) Dynamic Array integrated fluidic circuit (IFC) (49). ..

    Article Title: The Early Life Microbiota Is Not a Major Factor Underlying the Susceptibility to Postweaning Diarrhea in Piglets.
    Article Snippet: A total of 500 ng RNA purified with Direct-zol RNA MiniPrep Plus kit (Zymo Research) were retrotranscribed into cDNA with the kit GoScript Reverse Transcription Mix, Random primer (Promega) as described previously (37). .. Gene expression was analyzed by real-time qPCR using Biomark microfluidic system using a Dynamic Array IFC for gene expression (Fluidigm) according to the manufacturer recommendations July/August 2023 Volume 11 Issue 4 10.1128/spectrum.00694-23 10 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 24 J an ua ry 2 02 4 by 1 03 .1 05 .3 4. ..

    Amplification:

    Article Title: Protective interplay: Mycobacterium tuberculosis diminishes SARS-CoV-2 severity through innate immune priming.
    Article Snippet: The obtained RNA was then utilized in the HighCapacity RNA-to-cDNA kit for cDNA synthesis using SuperScriptTM IV Reverse Transcriptase (Thermo Fisher Scientific), containing a reverse transcriptase with a high-fidelity enzyme following manufacture protocol. .. For Fluidigm Real-Time PCR and Dynamic Array IFC (Integrated Fluidic Circuit) Setup, specific target amplification (STA) was done as per the manufacturer’s recommendations as the initial step (pre-amplification of cDNA) for the Biomark HD system (Standard BioTools, formerly Fluidigm) carried out on the Standard BioTools 48.48 Gene Expression (GE) Dynamic Array integrated fluidic circuit (IFC) (49). ..



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